Determination of Oil Quality and Antifungal Effect of Selected Citronella Accessions (Cymbopogon nardus, Cymbopogon winterianus) to Formulate an Anti-Dandruff Shampoo

Citronella is an aromatic grass of the family Poaceae which can be classified into two categories Ceylon (Cymbopogon nardus) and Java winterianus). The oil was extracted from five selected (HP T1, HP T2 T3) (MP T1 MP T2) accessions using steam distillation hydrodistillation methods. quantity by with Xylene higher (2.45-2.67 mL/100 g) than (1.57-1.64 g). T1-5.52 %, T2- 1.40 T3- 1.05 %) T1- 1.25%, 1.79%) showed a significant difference (P<0.0001) there no (P=0.7055) between 1.07%, 1.18 1.19%) 1.16%, 1.23%) oils distillation. Both organoleptic properties pale yellow to brownish colour strong citrusy aroma meets ISO 3848 3849 standards. refractive index (1.465-1.487), relative density (0.893-0.910), ethanol solubility (1:2 mL) within ranges specified in SLS 170 exhibited (1.4660-1.4730), (0.880-0.892), mL), optical rotation (-5? 0?) specifications Geraniol, Citronellol, Citronellal were identified as major constituents gas chromatography-mass spectrometry (GC-MS) where high Geraniol content (48.60-49.17%) (16.93-26.49%). All types tested inhibition against Candida albicans T3 (1.9 cm) T1(2.0 highest promising antifungal activity among respectively. Therefore, these for antidandruff shampoo formulation. samples formulated 2% v/v concentrations determined MIC C. albicans. Antidandruff greater - 2.50.05 cm; crude 1.90.11 T1-2.00.1 cm), also attained physical such pH (4.0-8.0), foam height (>100 dirt dispersion (no ink foam), viscosity, low wetting time solid T3-14.750.12%; T2-12.330.19%) acceptable specification range. This study exhibits that has all accessions. Hydrodistillation used extract method both types. Compared oil, potential industrial applications Also, meet physiochemical requirements quality standards excellent albicans, provides prospective use natural, safe, eco-friendly fungicide future product formulations.